Web30 mag 2024 · DNA was extracted by phenol-chloroform-isoamyl alcohol (25:24:1) and ethanol precipitated in the presence of ammonium acetate and 60 µg glycogen. … Web(DSG) was introduced by restriction enzymes in-vitro within the coding sequence of the URA3 gene of this vector. To eliminate transfer artefacts, selection was first applied for the undamaged TRP1 gene followed by coun- ter selection for URA3 gene activity, which indicated cor- rect repair of the DSB and DSG.
ANTICORPI ANTI DS-DNA Centro Analisi
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