Putative endolysin

Author: twsp

August undefined, 2024

WebMar 1, 2024 · that a 36.1-kbp putative phage remnant region exists only on the genome of C. perfringens st13 and is absent from the genomes of the other sequenced C. perfringens strains (Figure S2). A gene designated psa (gene ID; CPE1138), encoding a putative endolysin, was detected at one end of this region; a putative holin gene is also present in … WebAug 6, 2016 · Endolysin from Staphylococcus aureus phage SA97 (LysSA97) was cloned and investigated. LysSA97 specifically lyse the staphylococcal strains and effectively disrupted staphylococcal biofilms. Bioinformatic analysis of LysSA97 revealed a novel putative cell wall binding domain (CBD) as well as two enzymatically active domains …

Endolysin of bacteriophage BFK20: evidence of a catalytic and a …

WebMay 7, 2024 · Dataset. We evaluated 2628 putative endolysin genes found in 183,298 genomic sequences of uncultured viral genomes previously identified by Fernández-Ruiz … WebApr 11, 2024 · Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A phiSM101 muramidase gene (psm) revealed by this study can be assumed to encode an N-acetylmuramidase, since the N-terminal catalytic domain deduced from the gene shows … clc lightspeed

Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin

WebMar 28, 2012 · Identification of putative cell wall binding domains. With only a single exception (Myrna gp243), the putative endolysin catalytic domain is flanked on its C … WebJul 21, 2024 · In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. … down syndrome association of the valley

Endolysin LysSTG2: Characterization and application to control

A Putative Amidase Endolysin Encoded by Clostridium perfringens …

WebMar 1, 2024 · Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. … WebClostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential … down syndrome association of greater toledoWebMar 10, 2016 · The PAC4 phage genome also has a 94 bp insert located at 15,793 bp in the putative endolysin encoding orf. This creates a stop codon, and divides this sequence into two orfs. While the product of one orf retains the region encoding an intact amidase domain, and is 98% homologous to a putative endolysin from P. clcl instruction

"WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the ... " - Putative endolysin

Putative endolysin

WebJan 31, 2024 · Putative endolysin 117 was the only successfully overexpressed endolysin, and it was renamed LyJH1892. LyJH1892 showed potent lytic activity against both methicillin-susceptible S. aureus and MRSA and showed broad lytic activity against coagulase-negative staphylococci. WebApr 12, 2011 · We found that an endolysin gene (psm) in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101 (Myers et al. 2006), encodes a …

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WebApr 11, 2024 · An ORF 161 consisting of 137 amino acids was annotated as a putative endolysin (GenBank accession QYC50633), namely LysJNwz. Conserved Domain analysis further revealed LysJNwz belongs to the L-Ala-D-Glu peptidase-like family of peptidase_ M15 superfamily with a single conserved domain between amino acids 9–136. WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, …

WebMay 25, 2024 · Bacteriophage encoded endolysin as an alternative antibiotics is a peptidoglycan degrading enzyme with a low rate of resistance. Here, the engineered endolysin was developed to defeat multiple drug-resistant (MDR) Acinetobacter baumannii. First, putative endolysin PA90 was predicted by genome analysis of isolated … WebNov 22, 2024 · In the present work, a putative endolysin (AbLys1) was identified and characterized. Ab Lys1 was evaluated for its antimicrobial activity against a clinically isolated A. baumannii strain. The work aimed at the discovery of new antimicrobial agents towards A. baumannii cells as an alternative non-antibiotic strategy to combat this …

WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum … WebMar 23, 2024 · To enable functional and structural studies of the putative Enc34 endolysin, the coding sequence of the ORF39 protein with a cleavable N-terminal hexahistidine-tag was cloned and expressed in ...

WebMethods: We propose a computational method for the prediction of endolysin and autolysin. First, a data set containing 27 endolysins and 41 autolysins is built. Then the protein is …

WebThe 723 putative endolysins ranged from 72 to 578 residues in length, using 24 different catalytic domains and 18 different cell wall-binding domains organized with 89 different … clclightsupply.comWebMar 1, 2024 · A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm ... clc living lab trailWebSep 1, 2024 · Identification and overexpression of recombinant endolysin LysSTG2. From the genome sequence of phage STG2, the predicted open reading frame for a putative endolysin gene encoding a protein (LysSTG2) consisting of 138 amino acids (expected molecular mass of 15.3 kDa) was identified. clcl is clipboard caching utilityWebNov 12, 2024 · In this study, the potential role of the VL1_T00003720 (denominated as Vl3720) gene, encoding a putative endolysin-like protein, has been investigated in the … clc lighting companyWebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This … down syndrome association trainingWebAug 1, 2011 · The putative endolysin gene (ORF24) previously determined in the phage BFK20 genome (EMBL accession no. AJ278322) had to be corrected from 576 bp (ORF24) to 813 bp (ORF24′) because sequencing errors were detected which resulted in a frameshift mutation. The endolysin of BFK20 (gp24′) contains 270 aa, which corresponds to a 30.1 … clc living centerWebThis study investigates 2,628 putative endolysin genes identified in 183,298 bacteriophage genomes. Previously, bioinformatic approaches resulted in a database of 66 SAR-endolysins. clc lighting